DNA
Delta1

Part:BBa_K3939000:Design

Designed by: Lingwei Ding   Group: iGEM21_Tianjin   (2021-09-29)


Delta sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are some other sequences which are similar to delta sites. As Saccharomyces cerevisiae has a strong ability of homologous recombination(HR), we suspect that yeasts may use these sequence similar to delta recover double strand breaks(DSBs).


Source

[1]Shi Shuobo,,Liang Youyun,,Ang Ee Lui & Zhao Huimin.(2019).Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae.. Methods in molecular biology (Clifton, N.J.)(), doi:10.1007/978-1-4939-9142-6_6.

References